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31.
Moritomo T Minami A Inoue Y Nakanishi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1149-1151
An automatic counting method was developed for fish blood cells using a fluorescent dye, 3, 3-dihexyloxacarbocyanine (DiOC (6)(3)), that selectively stain lipid bilayers in living cells. In the present study, the DiOC(6)(3) method was applied to quail (Cotumix cotumix japonica) blood cells. After quail blood cells were stained with DiOC(6)(3), absolute counts and relative proportions of erythrocytes, granulocytes, monocytes, and lymphocytes plus thrombocytes in whole blood were obtained by means of flow cytometry (FC). The number of each cell types by the FC was in good agreement with those counted microscopically. This method will offer new possibilities for routine blood cell counting for avian medicine. 相似文献
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以吉林省通榆县为例,构建了县域农业可持续发展的生态安全评价指标体系,采用均方差法确定了评价指标的权重,对研究区1985、1990、1995、1998、2001、2004年6个时段的农业生态安全进行了评价.从评价结果可以看出,近20年,通榆县农业生态安全呈现下降态势,安全等级处于Ⅲ~Ⅴ级,安全状态从敏感态下降到风险态.1998年是研究区农业生态安全变化的转折点,从1998年起,农业生态安全有所回升.在分析变化原因的基础上,提出农业可持续发展的生态安全建设对策. 相似文献
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近几年,一些村集体开始有意愿投入土地整治中,并规避政府主导的方式,以自主筹资并主导整治的整治行为出现。村集体是土地整治密切的主体,针对村集体的主导整治行为,以北京市丰台区C镇Z村为例,利用利益相关者理论,结合传统政府主导下土地整治模式,对Z村土地复垦案例中各个利益相关者的角色、权责和利益3方面进行对比分析,在此基础上说明村集体主导下的土地整治模式下存在的冲突,并以此为村集体主导的土地整治模式发展提出明晰利益相关者政府与村集体的定位,减少利益相关者对土地的依赖等相关的建议与思考。 相似文献
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Feng Liu Qinghui Ai Kangsen Mai Beiping Tan Hongming Ma Wei Xu Wenbing Zhang Zhiguo LiuFu 《Journal of the World Aquaculture Society》2008,39(4):500-509
A 30‐d feeding experiment was conducted in tanks to investigate the effects of four different binders (2% in microdiet) on the survival, growth performance, and specific activity of digestive enzymes in tongue sole, Cynoglossus semilaevis Günther, postlarvae (24 d after hatching, with initial average wet weight of 22.4 ± 6.65 mg [mean ± SD]). Five approximately isonitrogenous and isoenergetic microbound diets were formulated with carrageenan (Car), sodium carboxymethylcellulose (CMS), sodium alginate (SA), and gelatin (Gel) as binders, and a diet with no special binder as a control. A commercial diet (RQ Com., manufactured by Marubeni Nisshin Feed Co., Ltd., Chita, Japan) was used as another tested diet, and Artemia nauplii was used as live prey control. The results showed that the survival of the fish fed SA diet (31.3%) was significantly higher than that of fish fed CMS (21.8%), Car (10.8%), and control (21.8%) diets (P < 0.05), but significantly lower than that of the fish fed the live prey (54.5%) diet (P < 0.05). The results of growth followed the similar pattern as that of survival, although there were no significant differences in specific growth rate (SGR) among fish fed SA, CMS, Gel, the control, and RQ diets (7.2, 6.8, 7.0, 6.9, and 7.3% per day; P > 0.05). However, fish fed Car diet had a significantly lower SGR (5.9% per day) than fish fed other diets. Percentages of secreted amylase in fish fed artificial diets were not significantly different, but fish fed artificial diets had significantly higher (P < 0.05) secreted amylase than live prey group. Percentage of secreted trypsin in fish fed SA diet (54.9%) was relatively higher compared with other binders, and not significantly different from RQ Com. and live prey treatments. However, trypsin in fish fed Car diets (29.3%) was significantly lower than in other groups. Specific activities of alkaline phosphatase (AP) in the intestine and AP and leucine aminopeptidase N in brush border membranes in fish fed SA diet (264.6, 1882.8, and 187.2 mU/mg protein) were relatively higher compared with other binders. These results indicate that SA is one of suitable binders compared with carrageenan, CMS, and gelatin in microdiet of postlarval tongue sole. 相似文献
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用基于PCR的分子标记分析甘蓝型油菜抗感菌核病品系间的遗传距离 总被引:5,自引:1,他引:4
用离体叶接种法鉴定了甘蓝型油菜选系对菌核病的抗感性,结果表明绝大多数抗性品系与感病品种间的差异达到显著或极显著水平。以两种基于PCR的标记估计所选10 份材料间的遗传距离。19 个随机引物扩增出了26 条重复性很好的RAPD 标记,1 对依据植物R基因的NBS区段设计的简并引物扩增出了3 条多态性的带。依据29 个标记进行聚类分析,结果表明抗、感性材料在遗传距离为0.1206 处被明显地区分开。5份抗性材料中,宁RS—1 与中油821、中R888 等其它抗性品系的遗传距离较远 相似文献
38.
Jiqiu Li Beiping Tan Kangsen Mai Qinhui Ai Wenbing Zhang Zhiguo Liufu Wei Xu 《Journal of the World Aquaculture Society》2008,39(4):477-489
A 63-d feeding experiment was conducted to determine the effects of dietary supplementation of probiotic bacterium Arthrobacter XE-7 on immune responses and resistance against Vibrio parahaemolyticus in the Pacific white shrimp, Litopenaeus vannamei . The probiotic bacteria were administered orally at four different doses of 0, 106 , 108 , and 1010 colony-forming unit (CFU)/g feed for shrimp. On Day 50, the shrimp were challenged with Vibrio parahaemolyticus by bath. On Days 7, 21, 49, and 63, six shrimp per tank were sampled to take intestine and hemolymph. With increasing dietary supplementation of probiotic bacteria, shrimp mortality decreased from 63.16% (the control) to 55.9% (106 CFU/g feed), 51.75% (108 CFU/g feed), and 51.78% (1010 CFU/g feed), respectively. Vibrio counts in intestine of shrimp fed probiotic bacterium Arthrobacter XE-7 was generally lower than that in the control shrimp ( P < 0.05). The probiotic bacteria generally increased the immune parameters in shrimp, that is, total hemocyte counts, percentage phagocytosis, respiratory burst activity, and serum phenoloxidase activity. The results showed that probiotic bacterium Arthrobacter XE-7 can be used as probiotic in shrimp feed. 相似文献
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